Focus Marzo 2015 Pdf W2
Unlimited Form W2 and W3 Printing No red forms are needed to print W-2 and W-3 for recipients and SSA. Our ezW2 software can with your laser or ink-jet printer. The black and white substitute forms of W-2 Copy A and W-3 are SSA-approved.If you prefer the pre-printed forms, ezW2 software also.Unlimited Form 1099-misc and 1096 Printing. Recipient copyOur ezW2 1099 Software can Copy 1, 2, B, C on white paper.
IRS copyIRS does not certify the substitute forms. If you will mail the paper forms to IRS, the red forms are required to fill out 1099-misc Copy A and 1096. PDF Forms (Optional)If you want to cut the mailing cost, ezW2 software can generate PDF format forms for W2 copies B, C, D, 1 and 2 and 1099-misc copy 1, 2, B and C.Electronic Filing (Optional)Our ezW2 software can generate and that you can upload to IRS and SSA Sites.Easy-to-use, Affordable and Free Trial Version. From $39. Supports unlimited W2 & 1099 recipients/payers.
Supports unlimited companies. Quick Data Import. Data rollover from previous years. Buy Now & Receive Key ImmediatelyFREE download. No credit card is needed,no obligation and totally risk free!ezW2 works on Windows 10ezW2 works on Windows Vista/7/8/8.1/10, 32-bit/64-bit system.New File Dates!!!. More on.Key Features of ezW2 2018, 1099-MISC & W-2 Print and Efile SoftwareFeaturesSmall Business Version$39 per installationEnterprise Version$79 per installationMultiple-user Network Versionfrom $99Print W2 FormsezW2 application is approved by SSA to print all W2 copies on White paper. Step 4: Enter 1099 informationThis is the form to Add/Edit 1099 data- The payer information will be filled automatically.- Once you select the contractor from the contractor list, the recipient information will be filled automatically.- Fill in the boxes on the 1099 Form.- Click the Question icon or the instructions link to get help.- Click the 'save' button to save the 1099 information.- Click the 'Print 1099 Forms' button to print the different 1099 Copies for this recipient.- Click the 'Print Instructions' button to print 1099 instructions.
EzW2 is very user-friendly and easy-to-use. We intentionally engineered this software for those end-users who are not professional accountants and payroll tax experts. We believe small business software should be simple - stupid simple - so that owners can focus their time and energy on running their businesses, not trying to figure out how to run software.ezW2 somes with sample database. Once you install this software, you are ready to generate your forms for testing purpose. Please feel free to.
Form W-2, Wage and Tax Statement, is used in the United States income tax system as an information return to report wages paid to employees and the taxes withheld from them. The form is also used to report FICA taxes to the Social Security Administration. Relevant amounts on Form W-2 are reported by the Social Security Administration to the Internal Revenue Service.Employers must complete a Form W-2 for each employee to whom they pay a salary, wage, or other compensation as part of the employment relationship. The Form W-2 reports income on a calendar year (January 1 through December 31) basis, regardless of the fiscal year used by the employer or employee for other Federal tax purposes.The form consists of six copies:. Copy A - Submitted by the employer to the Social Security Administration. (In addition, the employer must also submit Form W-3, which is a summary of all Forms W-2 completed, along with all Copies A submitted.
A W-3 Form is a transmittal form which is sent to the Social Security Administration (SSA) showing total earnings, Social Security wages, Medicare wages and withholding for all employees for the previous year.The W-3 is required to be sent to the SSA no later than the beginning of March, along with copies of all W-2 forms for all employees. The totals for each item on the W-3 must equal the totals for all of these items on all employee W-2 forms. The W-3 also includes total state wages and withholding for all employees.
Employers must file Form W-2 for wages paid to each employee from whom:. Income, social security, or Medicare tax was withheld or. Income tax would have been withheld if the employee had claimed no more than one withholding allowance or had not claimed exemption from withholding on Form W-4, Employee's Withholding Allowance Certificate.Also, every employer engaged in a trade or business who pays remuneration for services performed by an employee, including noncash payments, must file a Form W-2 for each employee even if the employee is related to the employer.
The drug target profile proposed by the Medicines for Malaria Venture for a malaria elimination/eradication policy focuses on molecules active on both asexual and sexual stages of Plasmodium, thus with both curative and transmission-blocking activities. The aim of the present work was to investigate whether the class of monovalent ionophores, which includes drugs used in veterinary medicine and that were recently proposed as human anticancer agents, meets these requirements.
The activity of salinomycin, monensin, and nigericin on Plasmodium falciparum asexual and sexual erythrocytic stages and on the development of the Plasmodium berghei and P. Falciparum mosquito stages is reported here. Gametocytogenesis of the P. Falciparum strain 3D7 was induced in vitro, and gametocytes at stage II and III or stage IV and V of development were treated for different lengths of time with the ionophores and their viability measured with the parasite lactate dehydrogenase (pLDH) assay. The monovalent ionophores efficiently killed both asexual parasites and gametocytes with a nanomolar 50% inhibitory concentration (IC 50).
Salinomycin showed a fast speed of kill compared to that of standard drugs, and the potency was higher on stage IV and V than on stage II and III gametocytes. The ionophores inhibited ookinete development and subsequent oocyst formation in the mosquito midgut, confirming their transmission-blocking activity. Potential toxicity due to hemolysis was excluded, since only infected and not normal erythrocytes were damaged by ionophores. Our data strongly support the downstream exploration of monovalent ionophores for repositioning as new antimalarial and transmission-blocking leads. INTRODUCTIONIn 2012, malaria caused 627,000 deaths, and there were 207 million reported cases.
Among the five species that infect humans, Plasmodium falciparum is responsible for the majority of deaths and severe cases. The recommended malaria control measures include drug treatment, in particular with artemisinin-based combination therapy (ACT), and protection from the vectors with insecticide-treated bed nets and indoor residual spraying. However, the effectiveness of control tools is seriously threatened by the emergence and spread of drug and insecticide resistance. Even for artemisinins, which until now were safe and effective, resistance is a growing issue in Asia, particularly on the Cambodia-Thailand border, which is the cradle of antimalarial resistance (, ). New drugs and new lead compounds for antimalarial drug development are greatly needed.In the past few years, the international strategy against malaria has changed toward malaria elimination and, ultimately, eradication. The Medicines for Malaria Venture (MMV) has defined four target candidate profiles (TCPs) that describe the requirements for novel tools for the control and elimination of malaria.
In particular, new multistage antimalarial drugs able to kill the liver or sexual stages of the parasite and/or that are capable of preventing the parasite development in the mosquito are needed (, ). Gametocytes are the sexual stage of Plasmodium, which appear concomitantly in the circulation or after the asexual intraerythrocytic stage. They undergo five stages of maturation, from I to V. Stage V gametocytes, when taken up by Anopheles mosquitoes during a blood meal, become gametes and fuse to form a zygote. Subsequently, the zygote transforms into a motile ookinete and becomes an oocyst, which divides to produce sporozoites that are ready to restart the cycle.
As the stage responsible for transmission, gametocytes are an essential target for malaria elimination/eradication, and the identification of gametocytocidal compounds has become an absolute priority.The strategy of drug repositioning (the usage of existing drugs for new therapeutic indications) allows a significant reduction in development costs, time to market, and risks of failure. This is particularly important for diseases in developing countries, for which research funds are limited. Here, we provide evidence in support of the repurposing of salinomycin and/or other ionophores as antimalarial and transmission-blocking agents.
Salinomycin is a polyether antibiotic isolated from Streptomyces spp. And a monovalent ionophore for alkali ions with relative K + selectivity, able thus to interfere with mitochondrial functions.
Salinomycin was patented in 1974 as an anticoccidial agent and has been used since then in poultry and other livestock. More recently, salinomycin and other ionophores, such as monensin and nigericin (sodium and potassium antiporters, respectively), were found to inhibit cancer stem cell growth by modulating the Wnt pathway (,). This finding has prompted the usage of salinomycin for compassionate use in a few cancer patients, with promising results.Salinomycin and other ionophores (gramicidin, lasalocid, and monensin) have already been reported to be active against P.
Falciparum parasites (,), but the potency of this class of compounds as transmission-blocking agents has not yet been fully investigated. The aim of the present work was to evaluate the antimalarial activity of salinomycin, monensin, and nigericin on both the asexual and transmission stages of P. Falciparum cultures.P. Falciparum cultures were carried out according to the method of Trager and Jensen , with minor modifications. Falciparum strains used in this study are either CQ sensitive (D10, 3D7, and the Ghana isolate) or CQ resistant (W2 and the Burkina Faso isolate). The resistance profile of W2 is well documented in the literature , whereas the P.
Falciparum Burkina Faso isolate is considered resistant, since its 50% inhibitory concentration (IC 50) of chloroquine is 100 nM, which is commonly accepted as the threshold for resistance. All the strains were maintained at 5% hematocrit (human type A-positive red blood cells for D10, W2, and the Burkina Faso and Ghana isolates and O-positive red blood cells for 3D7) in RPMI 1640 medium containing 24 mM sodium bicarbonate (EuroClone; Celbio), with the addition of 0.01% hypoxanthine, 20 mM HEPES, and 2 mM glutamine. All the parasites were cultured in the presence of 1% AlbuMAX II (lipid-rich bovine serum albumin), except the 3D7 strain, which was cultured in the presence of 10% (vol/vol) naturally clotted heat-inactivated O+ human serum (Interstate Blood Bank, Inc.), which ensures constant and high gametocyte production.
Focus Marzo 2015 Pdf W2 Template
All the cultures were maintained at 37°C in a standard gas mixture consisting of 1% O 2, 5% CO 2, and 94% N 2. Falciparum Ghana and Burkina Faso isolates derived from two different patients with malaria admitted to the Ospedale Sacco (Milan, Italy) returning to Milan from Ghana or Burkina Faso, respectively. The parasites were adapted to grow in culture and are not clonal strains.Gametocytogenesis was triggered as previously described.
Falciparum 3D7 asexual parasite cultures were diluted to 0.5% parasitemia, and the medium was changed daily without the addition of fresh red blood cells (RBC). When a parasitemia of 5% was obtained and the parasites were stressed by nutrient deprivation, the cultures were treated for 48 to 72 h with N-acetylglucosamine (NAG) (Sigma-Aldrich) to clear residual asexual parasites. Stage II and III gametocytes were obtained and used for the experiments after 4 days after the addition of NAG to the culture, while stage IV and V gametocytes were used after 7 to 8 days. Gametocyte stages were routinely checked in the Giemsa-stained smears.
Falciparum drug susceptibility assay.Salinomycin, monensin, and nigericin sodium salts (Sigma-Aldrich) were dissolved in either dimethyl sulfoxide (DMSO) (salinomycin and nigericin) or ethanol (monensin) and then diluted with medium to achieve the required concentrations (final DMSO or ethanol concentration of. In vitro mammalian cell toxicity assay.A long-term cell line of human dermal microvascular endothelial cells (HMEC-1) immortalized by simian virus 40 (SV40) large T antigen was kindly provided by the Centers for Disease Control and Prevention, Atlanta, GA, USA. The cells were maintained under standard conditions at 37°C in a 5% CO 2 incubator in MCDB 131 medium (Gibco-BRL, Paisley, Scotland) supplemented with 10% fetal calf serum (HyClone, Logan, UT, USA), 10 ng/ml epidermal growth factor (PeproTech, Rocky Hill, NY, USA), 1 μg/ml hydrocortisone (Sigma Italia, Milan, Italy), 2 mM glutamine (EuroClone, Pero, Italy), 100 U/ml penicillin, 100 mg/ml streptomycin (EuroClone), and 20 mM HEPES buffer (pH 7.3) (EuroClone). For the toxicity experiments, HMEC-1 cells at 1.0 × 10 4 cells/100 μl/well were plated in 96-well plates and incubated at 37°C and 5% CO 2 overnight.
The cells were then treated for 72 h with different doses of salinomycin, monensin, or nigericin diluted as described above (final volume, 200 μl/well). Three independent experiments were performed in duplicate. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) (Sigma) cytotoxicity assay was used to measure cell viability, as described elsewhere.
2016 W2 And W3 Forms
Cytotoxicity was expressed as the 50% inhibitory concentration (IC 50). IC 50 and selectivity index.The results of the chemosensitivity assays were expressed as the percent viability compared to the untreated controls, calculated with the following formula: 100 × (OD of treated sample − blank/OD of untreated sample − blank) (OD, optical density). As a blank, uninfected RBCs were used. The percent viability was plotted as a function of drug concentrations, and the curve fitting was obtained by nonlinear regression analysis using a four-parameter logistic method (software Gen5 1.10 provided with the Synergy4 plate reader Biotek). The IC 50 was extrapolated as the dose that induced a 50% inhibition of gametocyte viability.The selectivity index (SI) was calculated to evaluate the toxicity impact of salinomycin, monensin, or nigericin against normal human cells compared to the toxicity against the parasite, and this allows an assessment of the selectivity of these drugs for the parasite. The SI was calculated as the ratio between the cytotoxic IC 50s against HMEC-1, calculated as previously described, and the parasitic IC 50s against 3D7 gametocytes at stages IV and V for both the time points 72 h and 72 plus 72 h. Time course experiments.Two different time course protocols were employed.
In the first case, stage IV and V gametocytes were incubated with salinomycin, monensin, or nigericin at 1, 10, or 100 nM for different lengths of time (2, 6, 24, 48, or 72 h), and the pLDH assay was performed at the end of each treatment.In the second series of experiments, stage IV and V gametocytes were incubated with salinomycin at 1, 10, or 100 nM for different lengths of time (2, 6, 24, 48, or 72 h), the drugs were removed, fresh medium was added, the plate was centrifuged, and the medium was changed again. The pLDH assay was performed 72 h after the onset of the experiment, i.e., 72 h after the addition of the drugs to the cultures.For both experimental schemes, two independent experiments were performed in triplicate, and the results were expressed as the percent viability compared to that of the untreated controls. Hemolysis.Gametocyte cultures (about 0.5 and 1% parasitemia), fresh RBCs, or RBCs kept for 10 days under the same culture conditions as the gametocytes were diluted to 1% hematocrit and treated for 72 h with different doses (1, 10, and 100 nM) of salinomycin, monensin, or nigericin.
Hemolysis was evaluated by measuring spectrophotometrically the release of hemoglobin in the supernatants (absorbance at 405 nm, Soret band) and calculating the ratio of untreated to ionophore-treated samples. Similar experiments were performed on mouse RBCs. Blood from uninfected or Plasmodium berghei-infected mice, diluted to 7.5% hematocrit, was incubated for 24 h at 19°C with monensin and salinomycin at doses ranging from 1 to 50 nM. Hemoglobin release in the well supernatants was measured as described above.The percentages of hemolysis for both human and mouse RBCs were estimated by referring to a standard curve prepared with serially diluted RBCs, which were lysed with saponin (1%). Early sporogonic development P. Berghei assay.P.
Berghei CTRPp.GFP, a strain expressing the green fluorescent protein (GFP) exclusively at early sporogonic stages (zygotes, ookinetes, and early oocysts), was used (kindly provided by R. Sinden, Imperial College, London, United Kingdom) to infect BALB/c mice and recover P.
Berghei gametocytes for the early sporogonic development assay. Experimental animal rearing and handling were in compliance with the Italian legislative decree on the protection of animals used for experimental and other scientific purposes and in full adherence with the European Directive 2010/63/UE.The early sporogonic development assay was performed according to the protocol developed by Delves and colleagues , with slight modifications. Salinomycin and monensin were dissolved in DMSO and ethanol, respectively, and then diluted further to obtain the desired concentrations with ookinete medium (RPMI 1640 containing 25 mM HEPES, 25 mM sodium bicarbonate, 50 mg/liter hypoxanthine, 100 μM xanthurenic acid pH 7.6 to 8, supplemented with 20% heat-inactivated fetal bovine serum, 50 U/ml penicillin, and 50 μg/ml streptomycin). All chemicals were purchased from Sigma-Aldrich. Aliquots (20 μl) of compounds at a concentration 10 times higher than the desired test concentrations were then added to the microplate wells (96-well plates; Nunc, Denmark) containing 165 μl of ookinete medium. Subsequently, 15 μl of gametocytemic blood obtained by cardiac puncture from P. Berghei CTRPp.GFP-infected mice were added and the plates incubated at 19°C.
DMSO and ethanol at 0.3% were used as solvent controls. After 24 h of incubation, the well contents were diluted (1:550) in another plate, and the zygotes and ookinetes expressing green fluorescent protein were enumerated with the help of an ocular grid with a fluorescence microscope (×400 magnification; Zeiss). Compounds were tested in triplicate in two independent experiments using different gametocyte donor mice. The data are expressed as the percent inhibition of zygote and ookinete formation in drug-treated samples compared to that of the controls. Luminescent standard membrane feeding assays.Compounds were serially diluted in DMSO and subsequently in parasite culture medium to reach a final DMSO concentration of 0.1%. Luminescent standard membrane feeding assays were performed essentially as described by Stone et al.
, with the exception that we used an hsp70 promoter instead of the elongation factor 1 alpha (EF-1α) promoter to drive the expression of the luciferase reporter gene (M. Van Gemert, B. Van Schaijk, R. Sauerwein, T. Bousema, and K. Dechering., unpublished data). Luminescence intensities were analyzed for 24 mosquitoes per sample.
To determine the background luminescence levels, 24 uninfected mosquitoes were analyzed in parallel. For the determination of oocyst prevalence, mosquitoes were considered infected when the luminescence activity was 5 standard deviations above the average luminescence level observed in the uninfected mosquitoes. Ionophores inhibit asexual P.
Falciparum growth.Salinomycin, monensin, and nigericin were tested in vitro for antiplasmodial activity against three CQ-sensitive (D10, 3D7, and the Ghana isolate) and two CQ-resistant (W2 and the Burkina Faso isolate) strains of P. Falciparum using CQ and DHA as reference drugs. All the ionophores displayed a strong inhibition of asexual parasite growth against all the tested strains. The activity of monensin (IC 50 range, 0.5 to 1.0 nM) and nigericin (IC 50 range, 1.8 to 1.9 nM) against P. Falciparum laboratory strains was superior to that of DHA (IC 50 range, 2.1 to 5.6 nM). Against the CQ-sensitive parasites, the potency of salinomycin was slightly lower than that of CQ (IC 50 range, 22 to 40 versus 11 to 19 nM CQ), while monensin and nigericin were approximately 10-fold more active than CQ.
Differently from CQ, all the ionophores showed low-nanomolar activity against the W2 and Burkina Faso strains, which indicates the absence of cross-resistance. Ionophores inhibit P.
Falciparum gametocyte viability.Using the pLDH assay, we were able to calculate the IC 50 for all the ionophores, even after the first 72 h of incubation. The ionophores are far more potent than the reference compound epoxomicin or DHA. Neither compound induced a 50% reduction in gametocyte viability at the 72-h time point, even at the highest concentration tested, but required an additional 72 h without drugs to obtain a 50% inhibition of viability and thus to calculate the IC 50. Therefore, the ionophores appear to be the most potent compounds tested so far in our assay, in that they reduce gametocyte viability over a very short period, with an IC 50 in the nanomolar range. DrugGametocyte stagesIC 50 (nM) for incubation for (h) a:SI for incubation for (h) b:HMEC-1 IC 50 (nM) a7272 + 727272 + 72SalinomycinII–III29.7 ± 9.2 c14.5 ± 7.4 d157.5 ± 45.0IV–V13.8 ± 1.46.3 ± 1.711.425.0MonensinII–III4.6 ± 1.01.9 ± 1.3169.6 ± 48.0IV–V6.1 ± 1.15.7 ± 1.127.829.8NigericinII–III10.6 ± 4.02.7 ± 1.286.4 ± 31.0IV–V9.3 ± 4.90.9 ± 0.49.396.0DHAII–III50014.3 ± 8.73,094.8 ± 316.5IV–V500156.3 ± 78.3NA e19.8EpoxomicinII–III10012.1 ± 4.511.7 ± 3.1IV–V1006.7 ± 1.9NA1.7. ENA, not applicable.Selective chemosensitivity of gametocytes in different phases of their development was previously described. When the IC 50 of salinomycin was determined against stage II and III young gametocytes or stage IV and V mature gametocytes, the results were significantly different.
After 72 h of treatment, salinomycin appeared to be more effective (lower IC 50) on mature than on young gametocyte stages. After 72 plus 72 h of incubation, the differences between stages II and III and IV and V remained significant. Monensin and nigericin did not show a significant difference in activity against young versus mature gametocytes.The IC 50 of the ionophores on human endothelial cells (HMEC-1) was lower than that of DHA, suggesting potential toxicity. However, due to the higher activity on mature gametocytes, the selectivity indices of salinomycin and monensin (25 and 29.8, respectively) are comparable to that of DHA (19.8), and that of nigericin was even higher (96.0).To investigate the time to kill, time course experiments were performed by treating gametocytes with different doses of compounds for various pulse-inhibitory periods (2, 6, 24, 48, and 72 h) ( and ). When the pLDH activity was evaluated immediately after salinomycin treatment , the gametocyte viability was reduced by 41% with the 100 nM dose only and with a minimum incubation time of 24 h. Lower doses were ineffective.
Complete inhibition was observed after 48 h. The time-dependent inhibition of gametocyte viability by monensin or nigericin was similar to that of salinomycin at 100 nM, at a higher rate than with the 10 nM dose (at 72 h, inhibition of gametocyte viability was 27% for salinomycin, 68% for monensin, and 88% for nigericin). Time course of the activity of salinomycin, monensin, or nigericin on mature gametocytes. Gametocytes were incubated with the ionophores at 1 nM (circles), 10 nM (triangles), or 100 nM (squares) for different lengths of time (2, 6, 24, 48, and 72 h). (a) The pLDH viability assay was performed at the end of each incubation time. (b) The compounds were removed after each incubation time, fresh medium was added, and the pLDH assay was performed at 72 h. The results are the means ± SD from two independent experiments in triplicate.In a subsequent series of experiments, the drugs were removed after the different pulse-inhibitory periods, fresh medium was added, and the incubation was continued up to 72 h, when the pLDH activity was evaluated.
The results showed that salinomycin at 100 nM already inhibited pLDH activity by 65% after 2 h, whereas the same percentage of inhibition was obtained at the 10 nM dose only after 24 h. In this set of experiments, for each dose, the maximal inhibition was observed at 24 h, without any further increase up to 72 h.
The activity of monensin and nigericin was similar to that of salinomycin, with 61% and 82% inhibition, respectively, already after 2 h at the 100 nM dose. Hemolytic effect of ionophores is limited to infected RBCs at doses higher than the IC 50.To study the possible toxic effects of ionophores on RBCs, hemolysis was measured spectrophotometrically by determining the hemoglobin release in the supernatants of infected RBCs and, for comparison, of fresh uninfected RBCs or cultured uninfected RBCs. The cells were treated with different doses of ionophores for 72 h. The percentage of hemolysis induced on fresh RBCs was. Ionophores inhibit early sporogonic P.
Berghei development in vitro.Using an early sporogonic stage-specific GFP reporter strain of the rodent parasite P. Berghei (CTRPp.GFP), the capacity of gametocytes to undergo in vitro early sporogonic development (gamete formation, zygote formation, and ookinete maturation) was examined in the presence of the ionophores.As illustrated in, early sporogonic development was inhibited by both ionophores in a dose-dependent manner. Inhibition of ≥90% was recorded for salinomycin at 80 nM and for monensin at 40 nM. From a comparison of the IC 50s, it appeared that monensin (IC 50, 16.8 ± 2.5) was about twice as active as salinomycin (IC 50, 34.9 ± 5.1). Salinomycin and monensin block early sporogonic-stage development of P. Berghei in vitro.
The figure illustrates the mean percentages of inhibition and standard deviations for early sporogonic stages (zygotes and ookinetes) by salinomycin and monensin as a function of the compound concentration indicated on the x axis. The data are from 2 or 3 replicate experiments in triplicate wells, except for the 5 nM and 200 nM doses, which are based on a single experiment.To assess the toxicity of ionophores on infected and uninfected mouse RBCs, the release of hemoglobin was measured in wells incubated for 24 h with monensin or salinomycin at concentrations ranging from 1 to 50 nM. No appreciable hemolytic activity (. Ionophores block P. Falciparum oocyst development.P. Falciparum stage V gametocytes of an hsp70-GFP:luc reporter strain were incubated with compounds for 24 h and subsequently fed to Anopheles stephensi mosquitoes in the standard membrane feeding assays. Shows that salinomycin and monensin dose-dependently reduced oocyst intensity in the mosquito midgut, as indicated by a reduction in luciferase activity.
Both compounds showed IC 50s in the low-nanomolar range (IC 50, 1.9 nM for both drugs). The reduction in oocyst intensity translated into a reduction in oocyst prevalence or the number of infected mosquitoes ( and ). The IC 50 of monensin was 1.3 nM and that of salinomycin was 18 nM. These data indicate that monensin and salinomycin effectively block P. Falciparum transmission in the nanomolar range. Salinomycin and monensin block oocyst development in the mosquito. Falciparum stage V gametocytes of a transgenic hsp70-GFP::luc reporter strain were incubated for 24 h with different doses of compounds and fed to A.
Stephensi mosquitoes. (a and b) Luminescence intensities in individual mosquitoes as a function of the compound concentration indicated on the x axis. (c and d) Oocyst prevalence (percentage of infected mosquitoes) as a function of the compound concentration indicated on the x axis. DISCUSSIONRepositioning or repurposing of existing drugs for another application has already been proposed in the malaria field and applied to existing libraries against the asexual stages of P.
Successful repositioning of drugs significantly benefits the clinical development pipeline in term of costs and time. Here, we demonstrated the antimalarial and transmission-blocking activities of a specific class of compounds, the monovalent ionophores (i.e., salinomycin, monensin, and nigericin), polyether antibiotics that are used in several countries as anticoccidial agents in poultry. Despite being registered and FDA approved for veterinary use since 1974, only recently have the ionophores, and salinomycin in particular, experienced renewed interest as potential human anticancer agents (, ).Our data show that salinomycin, monensin, and nigericin are very active in vitro against a number of P. Falciparum isolates, including drug-resistant strains, in accordance with other reports (,).
Importantly, we also show that these molecules possess in vitro nanomolar activity against mature P. Falciparum stage IV and V gametocytes and against P. Berghei ookinetes, the earliest development stage in the mosquito vector. These data corroborate previous findings with salinomycin and monensin on P. Falciparum gametocytes after a large library screening. The cytocidal activity against gametocytes and ookinetes apparently leads to a functional block of oocyst development in the standard membrane feeding assays at concentrations in the nanomolar range.Monovalent ionophores are known for their activity as antifungal, antiparasitic, and antiviral agents, especially against multidrug-resistant pathogens.
Recent reports highlighted their activity against Trypanosoma brucei, Toxoplasma gondii, and cytomegalovirus (,). Considerable attention has been devoted recently to salinomycin and derivatives due to their selective activity against tumor stem cells (, ). A pilot study has been conducted in a few cancer patients, under compassionate use, and salinomycin was shown to induce tumor regression with minor side effects, which were lower than those of conventional chemotherapeutic drugs. In vitro, salinomycin has been shown to be active against multidrug-resistant cancer cells of different origins by inducing cell cycle arrest in G 1 phase , inhibiting cancer cell motility , modulating the autophagy process , or inducing leukemia cell apoptosis via the inhibition of Wnt signaling. The inhibition of Wnt signaling also seems to be involved in the inhibition of cytomegalovirus replication by salinomycin. Falciparum, the autophagy pathway is not yet completely characterized, but autophagy-related (ATG) proteins are abundantly expressed in P.
Falciparum gametocytes, suggesting that they may play a role in gametocytogenesis. Since monensin has been shown to induce autophagy and death of T. Gondii , we cannot exclude a similar effect of ionophores on P. Falciparum.Our data and reports in the literature confirm that the spectrum of action of monovalent ionophores against P. Falciparum is quite broad and not stage specific. Here, we demonstrate that ionophores inhibit the viability of stage IV and V gametocytes, as already described for all stages of asexual parasites.
In addition, there is evidence that salinomycin and monensin are effective against liver stages, both in vitro (HepG2 cells infected by P. Berghei sporozoites) and orally in P. Berghei-infected C57BL/6 mice in vivo (, ).
Unlike many reference drugs, like DHA (, ), salinomycin was more active on mature (stage IV and V) than young (stage II and III) gametocytes, which reinforces the transmission-blocking potential of this molecule. Mature gametocytes (stages IV and V) are metabolically quiescent (no protein synthesis or hemoglobin digestion) and thus relatively more resistant than early stage gametocytes or asexuals (, ). Only primaquine is effective in reducing late-stage gametocytemia in vivo. In that respect, the ionophores, and salinomycin in particular, compare quite well with the most advanced new antimalarial drug candidates of the MMV portfolio, including the spiroindolone KAE609 , the imidazolopiperazine KAF 156, and the quinolone-3-diarylether ELQ-300 (, ).
All these compounds have been shown to inhibit early and late P. Falciparum gametocytes at concentrations between 50 and 500 nM.Ionophores have a relatively fast speed of action.
More than 50% inhibition of gametocyte viability was seen at the first 72 h of the pLDH assay, whereas a longer incubation (72 plus 72 h) was necessary to observe the same effect with the reference drugs DHA, epoxomicin , and methylene blue. This is clearly related to the mode of action of the ionophores, but it also emphasizes the advantages of the dual-time pLDH assay, which discriminates between fast- and slow-acting compounds. The minimal contact time required to achieve 50% inhibition of gametocyte viability was 24 h for salinomycin and monensin at 10 nM and only 2 h for all three compounds at 100 nM. Indeed, salinomycin showed a similar time course activity on the intraerythrocytic parasite Babesia gibsoni, whereas 4 h was reported as the time for monensin to achieve 50% inhibition of asexual P.
Focus Workforce W2
Falciparum parasites (, ). As far as we know, an activity so fast has not yet been described for any other molecule.Due to the importance of ion balance for RBCs, one of the potential side effects of Na + and K + monovalent ionophores is the risk of hemolysis. However, few and controversial data are reported in the literature. Here, we show that a dose of ionophores close to the IC 50 for gametocytes (10 nM) was hemolytic only for gametocyte-infected but not uninfected normal human RBCs. Similarly, little hemolysis of uninfected mouse erythrocytes was observed after ionophore treatment in the concentration range of 10 to 25 nM, whereas on P.
Berghei-infected RBCs, a 6- to 8-fold increase in hemoglobin release in ionophore-treated compared to untreated samples was observed.These data highlight the specificity of ionophores for infected versus normal RBCs. Similar findings were reported by Gumila et al.
using asexual parasites and were attributed to the modification of the membrane properties of infected RBCs and the different phospholipid and lipid composition of parasite membranes. All ionophores strongly interact with phospholipid monolayers and facilitate the incorporation and transport of ions in low-cholesterol-containing membranes typical of asexual P.
Falciparum parasites (, ). Whether this may also apply to gametocyte-infected RBCs is not known yet.
Selectivity and antiparasitic activity might also depend on the ability of monovalent ionophores to induce K + efflux from the cytosol with a consequent increase in the influx of Na + and water. The parasite compartment is characterized by high K + and Ca 2+ levels and low Na + levels with respect to the infected RBC cytosol, which is poor in K + and rich in Na + (, ). This is different from normal RBCs, which are maintained at very high K + and low Na + concentrations by the Na +-K + ATPase pump.
Perturbation of the cation gradient and/or content in infected versus normal RBCs or in the parasite cytosol may be responsible for the selective antimalarial effects of ionophores. A similar mechanism has been proposed to explain the activity of salinomycin against T. Brucei and B.
Gibsoni (, ) and the egress of T. Gondii from host cells.To consider the potential use of ionophores as antimalarial drugs, it is of paramount importance to address clinical safety. In animals, monovalent ionophores can induce considerable neurotoxicity and cardiotoxicity after accidental exposure in nontarget species, such as calves, cats, and horses (,).
No appreciable risk for humans was demonstrated following the consumption of products from nontarget animals. However, preclinical pharmacological and safety data are scarce. Toxicity in humans has been reported in cases of accidental high-dose ingestion in people working with livestock (, ). In our assays, salinomycin appeared to be the most toxic for HMEC-1 cells among the ionophores tested, but the selectivity index was similar to that of the antimalarial drug DHA. Salinomycin exhibits an IC 50 similar to that of mefloquine when tested against HepG2 cells, whereas monensin showed no significant toxicity at the highest tested concentration (46 μM). The narrow therapeutic index of salinomycin as a potential anticancer drug has already boosted the development of chemically modified derivatives or innovative drug delivery systems (,), which should be considered in their future development as new antimalarials.The pharmacokinetic properties of salinomycin as an anticoccidial agent have been fully investigated in several animal species.
As a highly lipophilic drug, salinomycin is well absorbed and distributed throughout the plasma and tissues (especially fat in chickens). In mice, its oral and intravenous bioavailability was shown to be limited by the presence of the P-glycoprotein; at a dose of 1 mg/kg of body weight, the peak concentration was reached after 2 h, and the half-life was approximately 6 h. So far, no detailed information is available on salinomycin pharmacokinetic properties or plasma levels in humans. In a pilot study of metastatic cancer patients, the dose of 200 μg/kg of intravenous salinomycin was given every second day for 12 to 14 days; this protocol was well tolerated and led to partial clinical regression of heavily pretreated and therapy-resistant cancers. The suitability of long-term treatment might be an advantage for a transmission-blocking agent, since mature gametocytes persist in the blood for up to 2 weeks.
However, the limited absorption, distribution, metabolism, and excretion (ADME)/Tox data presently available in animal models suggest that new derivatives with longer half-lives and improved safety profiles need to be developed.In conclusion, our data suggest a strong and fast activity of monovalent ionophores against asexual stages and against mature gametocytes and ookinetes, resulting in a block of sporogonic development in the mosquito. These compounds, being active against several different P. Falciparum life stages, thus appear to be particularly attractive as potential curative and transmission-blocking agents, as advocated by the International Malaria Control Program and by the MMV. Kuhen KL, Chatterjee AK, Rottmann M, Gagaring K, Borboa R, Buenviaje J, Chen Z, Francek C, Wu T, Nagle A, Barnes SW, Plouffe D, Lee MC, Fidock DA, Graumans W, van de Vegte-Bolmer M, van Gemert GJ, Wirjanata G, Sebayang B, Marfurt J, Russell B, Suwanarusk R, Price RN, Nosten F, Tungtaeng A, Gettayacamin M, Sattabongkot J, Taylor J, Walker JR, Tully D, Patra KP, Flannery EL, Vinetz JM, Renia L, Sauerwein RW, Winzeler EA, Glynne RJ, Diagana TT.2014.KAF156 is an antimalarial clinical candidate with potential for use in prophylaxis, treatment and prevention of disease transmission. Antimicrob Agents Chemother 58:5060–5067.